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V. K. Gond ¹, R. K. Nirala ², Jaya Chandran ²

Affiliations
1 Department of Animal Husbandry and Veterinary Science, Tirhut College of Agriculture (R.A.U.) Dholi, Muzaffarpur Bihar, IN
2 Department of Veterinary Pharmacology and Toxicology, Bihar Veterinary College, Patna Bihar, IN

Abstract

In the present investigation, a comparative pharmacokinetics study of three commercial preparations of enrofloxacin (10%) was studied in five healthy female goats @5mg/kg b.wt. The samples of plasma were collected at different time interval i.e. 0.042, 0.083, 0.125, 0.333, 0.5, 0.75mn and 1, 1.5, 2, 3, 4, 6, 8, 10, 12 and 24 h after oral administration of drugs. Estimation of enrofloxacin was done by HPLC method. The selected pharmacokinetics parameters were calculated using one compartment open model. The drug was present significantly at lower concentration in brand II and brand III.As compared to brand I from 0.042 to 3 h except at 1 h, the brand II was significantly lower as compared to brand I only. From 4 - 10 h the concentrations of the drug in plasma did not show any significant difference between brand I, II and III. The therapeutic concentration (0.125 μg.ml-1) was maintained upto 12 h in brand I and brand III. The drug was only present in brand III at 24 h. The absorption half-life (t1/2 Ka) of brand III was significantly higher (0.83 ± 0.18 h) as compared to brand I (0.39 ± 0.06 h), but more or less similar to brand II (0.61 ± 0.11). Higher value of t1/2 Ka of brand III showed the slow absorption of the drug. The value of elimination half-life (t1/2 ) of brand III (4.33 ± 0.58h) was found to be significantly higher as compared to brand I (2.94 ± 0.16) and brand II (2.47 ± 0.22 h). Mean absorption time (MAT) of brand III (2.69 ± 0.58 h) was noted to be significantly higher as compared to brand I (1.06 ± 0.38) and brand II (0.55 ± 0.32 h). Brand I (4.24 ± 0.78 μg.ml-1) attained maximum concentration (Cmax) as compare to brand II (1.66 ± 0.23 μg.ml- 1), but non-significantly higher as compared to brand III (2.18 ± 0.32 μg.ml-1). Time to attain maximum concentration (tmax) of all the brands were noted to be non-significant with a mean of 1.70 ± 0.12, 1.60 ± 0.18 and 1.30 ± 0.12 h for brand I, II and III, respectively. Over all this supports its frequency of use in treating septicemia, respiratory tract, urinary tract, skin, soft tissue, bone joint infections etc. in goat and all the three brands of ENR substituted for each other.

Keywords

Pharmacokinetics, Enrofloxacin, Antibacterial, Brands, HPLC, Goats

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V. K. Gond ¹, R. K. Nirala ², Archana ², C. Jayachandran ²

Affiliations
1 Department of Animal Husbandry and Veterinary Science, Tirhut College of Agriculture (R.A.U.), Dholi, Muzaffarpur, Bihar, IN
2 Department of Veterinary Pharmacology and Toxicology, Bihar Veterinary College, Patna, Bihar, IN

Abstract

Antimicrobial therapy constitutes a major component of modern medical and veterinary practices. Enrofloxacin has been developed exclusively for veterinary. In the present investigation, five clinically healthy female goats of non-descript were used. Three commercial preparations of enrofloxacin (10%) were used@5mg/kg b.wt .The samples of plasma were collected at different time interval i.e. 0.042, 0.083, 0.125, 0.333, 0.5, 0.75, 1, 1.5, 2, 3, 4, 6, 8, 10, 12 and 24 h after IM administration of drugs. Estimation of enrofloxacin were done by HPLC method at the flow rate was 0.6 ml.min^-1,. Loop size was 200 ml, injection volume was 400 ml, the chart speed was 0.25 mm.min^-1 and the detector sensitivity was 2.000 A.U.F.S. The mobile phase comprised of acetonitrile : methanol : water (17 : 3 : 80 v/v/v) containing 0.4% phosphoric acid (85% v/v) and 0.4% triethylamine (v/v). The pH of mobile phase was 3 (approx). The drug is present significantly at a lower concentration in brand II (0.07 ± 0.02 μg.ml^-1) as compared to brand I (1.58 ± 0.41μg.ml^-1) at 0.042 h. Similarly, brand II show lower concentrations upto 12 h .The drug maintained its therapeutic concentration (³ 0.125 μg.ml^-1) upto 12 h in all the three brands. The value of absorption half-life (t_1/2 Ka) of brand I, II and III were noted to be non-significant with a mean of 0.31 ± 0.04, 0.66 ± 0.13 and 0.83 ± 0.31 h, respectively. Brand I showed rapid absorption as compared to brand II and III but statistically it is non-significant. Elimination half life (t_1/2 â) of brand I (3.10 ± 0.34 h) was found to be lower as compared to brand II and brand III (4.25 ± 0.71 and 3.84 ± 0.55 h, respectively), Mean residential time (MRT) of brand I, brand II and brand III were noted to be non-significant with a mean of 5.54 ± 1.13, 7.22 ± 0.86 and 6.52 ± 0.83 h, respectively. The values of mean absorption time (MAT) of brand I, brand II and brand III were noted to be non significant with a mean of 2.61 ± 0.98, 2.70 ± 0.92 and 2.61 ± 1.26 h, respectively. Maximum attainable concentrations (C_max) was found to be significantly lower in brand II (1.94 ± 0.16 μg.ml-1) as compared to brand I (5.35 ± 0.87 μg.ml^-1). However in case of time to reach maximum concentration (T_max) there was no significant difference between different brands.In conclusion,all these three brands of enrofloxacin interchangeable and substituted for each other.

Keywords

Pharmacokinetics, Enrofloxacin, Antibacterial, Brands, HPLC, Goats

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